Hau Thi Bich Nguyen

Los Alamos National Laboratory

Journal article Open access

Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions

by Hau Thi Bich Nguyen, Thomas Michael Groseclose, Antonietta Maria Lillo, Nileena Velappan, Yuliya A. Kunde, Lexy Amber Esmith Lujan and Jennifer Marie Zupancic

First online publication 11/19/2025

Scientific Reports, 15, 1, 40798

Journal article Open access

Recent advances in enzyme engineering for improved deconstruction of poly(ethylene terephthalate) (PET) plastics

by Thomas Michael Groseclose and Hau Thi Bich Nguyen

First online publication 08/20/2025

Communications Materials, 6, 1, 190

Journal article Open access

Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assay

by Thomas Michael Groseclose, Zoe Kyoko Taylor, Lexy Amber Esmith Lujan, Taraka T. Dale and Hau Thi Bich Nguyen

First online publication 07/15/2025

STAR Protocols, 6, 3, 103969

Journal article Open access

Engineering PHL7 for improved poly(ethylene terephthalate) depolymerization via rational design and directed evolution

by Thomas Michael Groseclose, Erin Anna Kober, Matilda Clark, Benjamin Moore, Ramesh Kumar Jha, Gregg T. Beckham, Andrew R Pickford, Taraka T. Dale and Hau Thi Bich Nguyen

First online publication 05/26/2025

Chem Catalysis, 101399

Journal article Open access

Machine Learning Framework for Conotoxin Class and Molecular Target Prediction

by Hau Thi Bich Nguyen, Duc P. Truong, Lyman Keegan Monroe and Robert F. Williams

First online publication 11/03/2024

Toxins, 16, 11, 475

Journal article Peer reviewed

A High-Throughput Screening Platform for Engineering Poly(ethylene Terephthalate) Hydrolases

by Thomas M. Groseclose, Erin A. Kober, Matilda Clark, Benjamin Moore, Shounak Banerjee, Victoria Bemmer, Gregg T. Beckham, Andrew R. Pickford, Taraka T. Dale and Hau B. Nguyen ... (11 authors)

Published 10/04/2024

ACS catalysis, 14, 19, 14622 - 14638

The ability of enzymes to hydrolyze the ubiquitous polyester, poly(ethylene terephthalate) (PET), has enabled the potential for bioindustrial recycling of this waste plastic. To date, many of these PET hydrolases have been engineered for improved catalytic activity and stability, but current screening methods have limitations in screening large libraries, including under high-temperature conditions. Here, we developed a platform that can simultaneously interrogate PET hydrolase libraries of 10(4)-10(5) variants (per round) for protein solubility, thermostability, and activity via paired, plate-based split green fluorescent protein and model substrate screens. We then applied this platform to improve the performance of a benchmark PET hydrolase, leaf-branch compost cutinase, by directed evolution. Our engineered enzyme exhibited higher catalytic activity relative to the benchmark, LCC-ICCG, on amorphous PET film coupon substrates (similar to 9.4% crystallinity) in pH-controlled bioreactors at both 65 degrees C (8.5% higher conversion at 48 h and 38% higher maximum rate, at 2.9% substrate loading) and 68 degrees C (11.2% higher conversion at 48 h and 43% higher maximum rate, at 16.5% substrate loading), up to 48 h, highlighting the potential of this screening platform to accelerate enzyme development for PET recycling.

Journal article Open access Peer reviewed

Engineering highly stable variants of Corynactis californica green fluorescent proteins

by Geoffrey S. Waldo, Thomas Charles Terwilliger, Li-Wei Hung and Hau Thi Bich Nguyen

Published 02/2024

Protein Science, 33, 2, e4886

Journal article Open access

Conotoxin Prediction: New Features to Increase Prediction Accuracy

by Hau Thi Bich Nguyen, Phan Minh Duc Truong, Lyman Keegan Monroe, Samantha Hiroshini Adikari, Zachary Joseph Sasiene, Paul W. Fenimore, Boian Alexandrov, Robert F. Williams and Jacob Miner

Published 11/2023

Toxins, 15, 11, 641

Journal article Open access

Engineering an efficient and bright split Corynactis californica green fluorescent protein

by Hau Thi Bich Nguyen, Geoffrey S. Waldo and Thomas Charles Terwilliger

Published 12/2021

Scientific Reports, 11, 1, 18440

Journal article Open access Peer reviewed

Rational design of antimicrobial peptides targeting Gram-negative bacteria

by Hau Thi Bich Nguyen, Loan Huynh, Goutam Gupta, Jeanette Velásquez, Roel Rabara and Supratim Basu

Published 06/2021

Computational Biology and Chemistry, 92, 107475

Hau Thi Bich Nguyen

Los Alamos National Laboratory

Output list