Jessica Zofie Kubicek-Sutherland

Los Alamos National Laboratory

Journal article Open access

Editorial: Towards the Rapid and Systematic Assessment of Vaccine Technologies

by Jessica Zofie Kubicek-Sutherland, Benjamin Hamilton Mcmahon, Evan Skowronski, Robert P. Webb and Traci Pals

First online publication 03/09/2026

Frontiers in Immunology, 1816160, 17

Journal article Open access

A consensus mathematical model of vaccine-induced antibody dynamics for multiple vaccine platforms and pathogens

by Ruy Miguel Ribeiro, Kristen Michelle Wilding, Carmen Molina-Paris, Jessica Zofie Kubicek-Sutherland, Benjamin Hamilton Mcmahon and Alan S. Perelson

First online publication 06/26/2025

Frontiers in Immunology, 16, 1596518

Journal article Open access

Prediction of Specificity of α-Conotoxins to Subtypes of Human Nicotinic Acetylcholine Receptors with Semi-supervised Machine Learning

by Hung Nguyen Do, Jessica Zofie Kubicek-Sutherland and Sandrasegaram Gnanakaran

First online publication 05/29/2025

ACS Chemical Neuroscience, 16, 12, 2196-2207

Journal article Open access

Methods integrating innate and adaptive immune responses in human in vitro immunization assays

by Tetiana Yuriyivna Bowley, Kiersten Danielle Lenz, Apoorv Shanker and Jessica Zofie Kubicek-Sutherland

First online publication 05/21/2025

Frontiers in Immunology, 16, 1584852

Journal article Open access

Diverse toxins exhibit a common binding mode to the nicotinic acetylcholine receptors

by Hung Nguyen Do, Jessica Zofie Kubicek-Sutherland and Sandrasegaram Gnanakaran

First online publication 02/26/2025

Biophysical Journal, 124, 8, 1195-1207

Journal article Open access

Reagent-free Hyperspectral Diagnosis of SARS-CoV-2 Infection in Saliva Samples

by Brandon Saint-John, Alejandro Wolf-Yadlin, Daniel Eric Jacobsen, Jamie L. Inman, Serge Gart, Matt Keener, Cynthia McMurray, Antoine M. Snijders, Harshini Mukundan and Jessica Zofie Kubicek-Sutherland ... (11 authors)

First online publication 01/16/2025

ECS Sensors Plus, 4, 1, 014601

Journal article Open access

Quantification of heterogeneity in human CD8+ T cell responses to vaccine antigens: an HLA-guided perspective

by Duane Charles II Harris, Apoorv Shanker, Makaela Morgan Montoya, Trent R. Llewellyn, Anna Rose Matuszak, Aditi Rajan Lohar, Jessica Zofie Kubicek-Sutherland, Ying Wai Li, Kristen Michelle Wilding and Benjamin Hamilton Mcmahon ... (14 authors)

First online publication 11/18/2024

Frontiers in Immunology, 15, 1420284

Journal article Open access

Nanodisc assembly from bacterial total lipid extracts

by Jessica Zofie Kubicek-Sutherland, Trent Riley Llewellyn, Kiersten Danielle Lenz, Olivia Raquel Chavez Pimentel and Makaela Morgan Montoya

First online publication 08/05/2024

Chemistry and Physics of Lipids, 264, 105425

Journal article Open access Peer reviewed

mRNA vaccine platforms to prevent bacterial infections

by Carson Bergstrom, Nicholas O. Fischer, Jessica Z. Kubicek-Sutherland, Zachary R. Stromberg and Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Published 06/01/2024

Trends in molecular medicine, 30, 6, 524 - 526

Journal article Open access Peer reviewed

Correlating transcription and protein expression profiles of immune biomarkers following lipopolysaccharide exposure in lung epithelial cells

by Daniel E Jacobsen, Makaela M Montoya, Trent R Llewellyn, Kaitlyn Martinez, Kristen M Wilding, Kiersten D Lenz, Carrie A Manore, Jessica Z Kubicek-Sutherland, Harshini Mukundan and Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Published 04/23/2024

PloS one, 19, 4, e0293680

Universal and early recognition of pathogens occurs through recognition of evolutionarily conserved pathogen associated molecular patterns (PAMPs) by innate immune receptors and the consequent secretion of cytokines and chemokines. The intrinsic complexity of innate immune signaling and associated signal transduction challenges our ability to obtain physiologically relevant, reproducible and accurate data from experimental systems. One of the reasons for the discrepancy in observed data is the choice of measurement strategy. Immune signaling is regulated by the interplay between pathogen-derived molecules with host cells resulting in cellular expression changes. However, these cellular processes are often studied by the independent assessment of either the transcriptome or the proteome. Correlation between transcription and protein analysis is lacking in a variety of studies. In order to methodically evaluate the correlation between transcription and protein expression profiles associated with innate immune signaling, we measured cytokine and chemokine levels following exposure of human cells to the PAMP lipopolysaccharide (LPS) from the Gram-negative pathogen Pseudomonas aeruginosa. Expression of 84 messenger RNA (mRNA) transcripts and 69 proteins, including 35 overlapping targets, were measured in human lung epithelial cells. We evaluated 50 biological replicates to determine reproducibility of outcomes. Following pairwise normalization, 16 mRNA transcripts and 6 proteins were significantly upregulated following LPS exposure, while only five (CCL2, CSF3, CXCL5, CXCL8/IL8, and IL6) were upregulated in both transcriptomic and proteomic analysis. This lack of correlation between transcription and protein expression data may contribute to the discrepancy in the immune profiles reported in various studies. The use of multiomic assessments to achieve a systems-level understanding of immune signaling processes can result in the identification of host biomarker profiles for a variety of infectious diseases and facilitate countermeasure design and development.

Jessica Zofie Kubicek-Sutherland

Los Alamos National Laboratory

Output list